The Ministry of External Affairs summoned US Charge d'Affaires Jason Meeks and lodged a strong protest over the attack on a tanker off Oman that left three Indian crew members missing.
No formal demarche was issued, but India had earlier condemned the attack without mentioning the perpetrator.
The tanker Settebello was reportedly targeted by US forces for trying to evade an American blockade of Iranian ports.
Of the 24 Indian crew on board, 21 have been rescued, and the Indian embassy in Oman is coordinating with Omani authorities in the search and rescue operation.
The MEA called for an immediate de-escalation of tensions and the restoration of free navigation and commerce through international waterways.